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KMID : 0881720150300030295
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Journal of Food Hygiene and Safety
2015 Volume.30 No. 3 p.295 ~ p.301
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Optimization of Culture Condition for Enhancing the Probiotics Functions
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Chang Bo-Yoon
Han Ji-Hye
Kim Sung-Yeon
Cha Bum-Suk
Ann Sung-Ho
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Abstract
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The functions of probiotics, particularly Lactic acid bacteria, have been studied in a range of human diseases, including cancer, infectious diseases, gastrointestinal disorders, and allergies. Among the many benefits associated with the consumption of probiotics, modulation of immune activity has received the most attention. This study aimed at investigating the improved immune stimulatory and stability of L. plantarum when cultivated on modified basal media supplemented with the Undaria pinnatifida co-cultured with L. plantarum. An in vitro test showed that U. pinnatifida media cultured L. plantarum is strong enough to survive in the gastric juice (gastric and bile acid). Mouse macrophage-derived cell lines RAW 264.7 was used to measured immune stimulating activity of L.plantarum. When U. pinnatifida media cultured by L. plantarum was NO and TNF-α production is significantly increased compared to basal media cultured L. plantarum. These results show that U. pinnatifida could be applied for a component for cultivation of L. plantarum. This optimized U. pinnatifida medium can be used the improving of stability and immune function on production of probiotics.
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KEYWORD
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probiotics, L. plantarum, Undaria pinnatifida, stability, immune
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